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dapi containing prolong diamond anti-fade  (Fisher Scientific)

 
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    Fisher Scientific dapi containing prolong diamond anti-fade
    Dapi Containing Prolong Diamond Anti Fade, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dapi+containing+prolong+diamond+anti-fade/dapi+containing+prolong+diamond+anti+fade/bio_rxiv__2020__07__27__223933-70-29-34
    Average 90 stars, based on 1 article reviews
    dapi containing prolong diamond anti-fade - by Bioz Stars, 2026-09
    90/100 stars

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    Article Snippet: Tissues were washed with pre-heated (∼48°C) buffer (20Mm Tris-HCL, 0.9M NaCl, 0.01% SDS, 0.05M EDTA) x 3 for 10 min (room temperature, dark) and mounted on glass slides with DAPI containing ProLong Diamond anti-fade (Fisher Scientific).



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    (A) HeLa cells were seeded onto <t>glass</t> <t>coverslips</t> and fixed using PFA, ice-cold methanol, or glyoxal. Reagent V was used to stain the cells, and <t>DAPI</t> was applied to stain the nuclei. (B) As in (A), but before fixation cells were treated with 0.5 mM H 2 O 2 for 10 min. All images were taken using a confocal microscope with identical laser intensity and settings across all samples. (C) HeLa cells were seeded as in (A) and fixed using PFA. Fixed samples were treated with either DNase or RNase, HgCl 2 , or hydroxylamine. ADP-ribosylation was visualised using Reagent V, and images were taken using confocal microscopy at the same settings for each sample. (D) Indicated metabolites were cross-linked to BSA using PFA and slot-blotted. Blots were incubated with the indicated antibodies and detected using chemiluminescence. Scale bars in (A, B, C) represent 10 µ M.
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    (A) HeLa cells were seeded onto <t>glass</t> <t>coverslips</t> and fixed using PFA, ice-cold methanol, or glyoxal. Reagent V was used to stain the cells, and <t>DAPI</t> was applied to stain the nuclei. (B) As in (A), but before fixation cells were treated with 0.5 mM H 2 O 2 for 10 min. All images were taken using a confocal microscope with identical laser intensity and settings across all samples. (C) HeLa cells were seeded as in (A) and fixed using PFA. Fixed samples were treated with either DNase or RNase, HgCl 2 , or hydroxylamine. ADP-ribosylation was visualised using Reagent V, and images were taken using confocal microscopy at the same settings for each sample. (D) Indicated metabolites were cross-linked to BSA using PFA and slot-blotted. Blots were incubated with the indicated antibodies and detected using chemiluminescence. Scale bars in (A, B, C) represent 10 µ M.
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    Image Search Results


    (A) HeLa cells were seeded onto glass coverslips and fixed using PFA, ice-cold methanol, or glyoxal. Reagent V was used to stain the cells, and DAPI was applied to stain the nuclei. (B) As in (A), but before fixation cells were treated with 0.5 mM H 2 O 2 for 10 min. All images were taken using a confocal microscope with identical laser intensity and settings across all samples. (C) HeLa cells were seeded as in (A) and fixed using PFA. Fixed samples were treated with either DNase or RNase, HgCl 2 , or hydroxylamine. ADP-ribosylation was visualised using Reagent V, and images were taken using confocal microscopy at the same settings for each sample. (D) Indicated metabolites were cross-linked to BSA using PFA and slot-blotted. Blots were incubated with the indicated antibodies and detected using chemiluminescence. Scale bars in (A, B, C) represent 10 µ M.

    Journal: Life Science Alliance

    Article Title: Protein and RNA ADP-ribosylation detection is influenced by sample preparation and reagents used

    doi: 10.26508/lsa.202201455

    Figure Lengend Snippet: (A) HeLa cells were seeded onto glass coverslips and fixed using PFA, ice-cold methanol, or glyoxal. Reagent V was used to stain the cells, and DAPI was applied to stain the nuclei. (B) As in (A), but before fixation cells were treated with 0.5 mM H 2 O 2 for 10 min. All images were taken using a confocal microscope with identical laser intensity and settings across all samples. (C) HeLa cells were seeded as in (A) and fixed using PFA. Fixed samples were treated with either DNase or RNase, HgCl 2 , or hydroxylamine. ADP-ribosylation was visualised using Reagent V, and images were taken using confocal microscopy at the same settings for each sample. (D) Indicated metabolites were cross-linked to BSA using PFA and slot-blotted. Blots were incubated with the indicated antibodies and detected using chemiluminescence. Scale bars in (A, B, C) represent 10 µ M.

    Article Snippet: After extensive washing in PBS and demineralised water, coverslips were mounted on microscopy slides using Prolong Anti-Fade Diamond Mountant containing DAPI (Thermo Fisher Scientific).

    Techniques: Staining, Microscopy, Confocal Microscopy, Incubation

    HeLa cells were grown on coverslips and fixed using either 3.7% PFA, ice-cold methanol, or glyoxal, followed by staining using the indicated antibodies and DAPI. Cells were imaged using confocal microscopy.

    Journal: Life Science Alliance

    Article Title: Protein and RNA ADP-ribosylation detection is influenced by sample preparation and reagents used

    doi: 10.26508/lsa.202201455

    Figure Lengend Snippet: HeLa cells were grown on coverslips and fixed using either 3.7% PFA, ice-cold methanol, or glyoxal, followed by staining using the indicated antibodies and DAPI. Cells were imaged using confocal microscopy.

    Article Snippet: After extensive washing in PBS and demineralised water, coverslips were mounted on microscopy slides using Prolong Anti-Fade Diamond Mountant containing DAPI (Thermo Fisher Scientific).

    Techniques: Staining, Confocal Microscopy